BD HorizonTM RB780 Mouse Anti-Human FoxP3

Description

Multicolor flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with BD Pharmingen Alexa Fluor 700 Mouse Anti-Human CD4 antibody Cat. No. 557922 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 followed by intracellular staining with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human FoxP3 antibody Cat. No. 568682/568683; Right Plot . The two-parameter pseudocolor density plot showing the correlated expression of CD4 versus FoxP3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with BD Pharmingen Alexa Fluor 700 Mouse Anti-Human CD4 antibody Cat. No. 557922 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 followed by intracellular staining with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human FoxP3 antibody Cat. No. 568682/568683; Right Plot . The two-parameter pseudocolor density plot showing the correlated expression of CD4 versus FoxP3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with BD Pharmingen Alexa Fluor 700 Mouse Anti-Human CD4 antibody Cat. No. 557922 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 followed by intracellular staining with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human FoxP3 antibody Cat. No. 568682/568683; Right Plot . The two-parameter pseudocolor density plot showing the correlated expression of CD4 versus FoxP3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with BD Pharmingen Alexa Fluor 700 Mouse Anti-Human CD4 antibody Cat. No. 557922 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 followed by intracellular staining with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human FoxP3 antibody Cat. No. 568682/568683; Right Plot . The two-parameter pseudocolor density plot showing the correlated expression of CD4 versus FoxP3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with BD Pharmingen Alexa Fluor 700 Mouse Anti-Human CD4 antibody Cat. No. 557922 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 followed by intracellular staining with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human FoxP3 antibody Cat. No. 568682/568683; Right Plot . The two-parameter pseudocolor density plot showing the correlated expression of CD4 versus FoxP3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with BD Pharmingen Alexa Fluor 700 Mouse Anti-Human CD4 antibody Cat. No. 557922 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 followed by intracellular staining with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human FoxP3 antibody Cat. No. 568682/568683; Right Plot . The two-parameter pseudocolor density plot showing the correlated expression of CD4 versus FoxP3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.