BD HorizonTM RB780 Mouse Anti-Human IgD

Description

Two-color flow cytometric analysis of IgD expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated overnight in complete tissue culture medium to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human IgD antibody Cat. No. 569140/569141; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of IgD expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated overnight in complete tissue culture medium to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human IgD antibody Cat. No. 569140/569141; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of IgD expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated overnight in complete tissue culture medium to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human IgD antibody Cat. No. 569140/569141; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of IgD expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated overnight in complete tissue culture medium to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human IgD antibody Cat. No. 569140/569141; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of IgD expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated overnight in complete tissue culture medium to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human IgD antibody Cat. No. 569140/569141; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of IgD expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated overnight in complete tissue culture medium to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human IgD antibody Cat. No. 569140/569141; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgD or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.