BD HorizonTM RB780 Mouse Anti-Human IgM

Description

Multicolor flow cytometric analysis of IgM expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human IgM antibody Cat. No. 569134/569135; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of IgM expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human IgM antibody Cat. No. 569134/569135; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of IgM expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human IgM antibody Cat. No. 569134/569135; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of IgM expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human IgM antibody Cat. No. 569134/569135; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of IgM expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human IgM antibody Cat. No. 569134/569135; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of IgM expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were incubated in complete medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human IgM antibody Cat. No. 569134/569135; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgM or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software.