BD HorizonTM RB780 Mouse Anti-Human KLRG1

Description

Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plots or BD Horizon RB780 Mouse Anti-Human KLRG1 antibody Cat. No. 569136/569137; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plots or BD Horizon RB780 Mouse Anti-Human KLRG1 antibody Cat. No. 569136/569137; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plots or BD Horizon RB780 Mouse Anti-Human KLRG1 antibody Cat. No. 569136/569137; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.