BD HorizonTM RB780 Mouse Anti-Human TCRalphabeta

Description

Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD19 antibody Cat. No. 563549/563551 , BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plots or BD Horizon RB780 Mouse Anti-Human TCRalphabeta antibody Cat. No. 569138/569139; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD19 antibody Cat. No. 563549/563551 , BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plots or BD Horizon RB780 Mouse Anti-Human TCRalphabeta antibody Cat. No. 569138/569139; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD19 antibody Cat. No. 563549/563551 , BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plots or BD Horizon RB780 Mouse Anti-Human TCRalphabeta antibody Cat. No. 569138/569139; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.