BD HorizonTM RB780 Mouse Anti-Human TIGIT

Description

Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with APC Mouse Anti-Human CD56 NCAM-1 Cat. No. 555518; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and BD Horizon BV421 Mouse Anti-Human CD45RO Cat. No. 562641/562649; Right Plots antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Top Plots or BD Horizon RB780 Mouse Anti-Human TIGIT antibody Cat. No. 569864/569940; Bottom Plots as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with APC Mouse Anti-Human CD56 NCAM-1 Cat. No. 555518; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and BD Horizon BV421 Mouse Anti-Human CD45RO Cat. No. 562641/562649; Right Plots antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Top Plots or BD Horizon RB780 Mouse Anti-Human TIGIT antibody Cat. No. 569864/569940; Bottom Plots as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leukocytes. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with APC Mouse Anti-Human CD56 NCAM-1 Cat. No. 555518; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and BD Horizon BV421 Mouse Anti-Human CD45RO Cat. No. 562641/562649; Right Plots antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Top Plots or BD Horizon RB780 Mouse Anti-Human TIGIT antibody Cat. No. 569864/569940; Bottom Plots as indicated. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the forward and side light-scatter characteristics of viable lymphocytes.