BD HorizonTM RB780 Mouse Anti-Mouse NK-1.1

Description

Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569231/569232; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569231/569232; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569231/569232; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569231/569232; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569231/569232; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 569231/569232; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.