BD HorizonTM RB780 Rat Anti-Human IL-2

Description

Two color flow cytometric analysis of IL-2 expression in activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Human IL-2 antibody Cat. No. 569130/569131; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two color flow cytometric analysis of IL-2 expression in activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Human IL-2 antibody Cat. No. 569130/569131; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two color flow cytometric analysis of IL-2 expression in activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Human IL-2 antibody Cat. No. 569130/569131; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two color flow cytometric analysis of IL-2 expression in activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Human IL-2 antibody Cat. No. 569130/569131; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two color flow cytometric analysis of IL-2 expression in activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Human IL-2 antibody Cat. No. 569130/569131; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two color flow cytometric analysis of IL-2 expression in activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mug/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Human IL-2 antibody Cat. No. 569130/569131; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.