BD HorizonTM RB780 Rat Anti-Human IL-6

Description

Multiparameter flow cytometric analysis of IL-6 expression in stimulated Human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 and Lipopolysaccharide LPS; Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465; Left Plot or BD Horizon RB780 Rat Anti-Human IL-6 antibody Cat. No. 570067/570148; Right Plot using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-6 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of IL-6 expression in stimulated Human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 and Lipopolysaccharide LPS; Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465; Left Plot or BD Horizon RB780 Rat Anti-Human IL-6 antibody Cat. No. 570067/570148; Right Plot using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-6 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of IL-6 expression in stimulated Human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 and Lipopolysaccharide LPS; Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465; Left Plot or BD Horizon RB780 Rat Anti-Human IL-6 antibody Cat. No. 570067/570148; Right Plot using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-6 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of IL-6 expression in stimulated Human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 and Lipopolysaccharide LPS; Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465; Left Plot or BD Horizon RB780 Rat Anti-Human IL-6 antibody Cat. No. 570067/570148; Right Plot using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-6 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of IL-6 expression in stimulated Human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 and Lipopolysaccharide LPS; Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465; Left Plot or BD Horizon RB780 Rat Anti-Human IL-6 antibody Cat. No. 570067/570148; Right Plot using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-6 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of IL-6 expression in stimulated Human peripheral blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma protein 10 ng/ml; Cat. No. 554617 and Lipopolysaccharide LPS; Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The PBMC were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465; Left Plot or BD Horizon RB780 Rat Anti-Human IL-6 antibody Cat. No. 570067/570148; Right Plot using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-6 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.