BD HorizonTM RB780 Rat Anti-Mouse CD127

Description

Multicolor flow cytometric analysis of CD127 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 555275 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD127 antibody Cat. No. 569065/569066; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD127 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD127 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 555275 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD127 antibody Cat. No. 569065/569066; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD127 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD127 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 555275 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD127 antibody Cat. No. 569065/569066; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD127 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD127 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 555275 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD127 antibody Cat. No. 569065/569066; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD127 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD127 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 555275 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD127 antibody Cat. No. 569065/569066; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD127 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD127 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 555275 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD127 antibody Cat. No. 569065/569066; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD127 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.