BD HorizonTM RB780 Rat Anti-Mouse CD16/CD32

Description

Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were stained simultaneously with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 569323/569324; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were stained simultaneously with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 569323/569324; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were stained simultaneously with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 569323/569324; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were stained simultaneously with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 569323/569324; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were stained simultaneously with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 569323/569324; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were stained simultaneously with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 569323/569324; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.