BD HorizonTM RB780 Rat Anti-Mouse CD25 (IL-2 Receptor alpha)

Description

Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 557308 and with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465 or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570356/570426; Middle Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot - BALB/c Mouse splenocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with BD Horizon RB780 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 557308 and with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465 or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570356/570426; Middle Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot - BALB/c Mouse splenocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with BD Horizon RB780 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 557308 and with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465 or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570356/570426; Middle Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot - BALB/c Mouse splenocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with BD Horizon RB780 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 557308 and with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465 or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570356/570426; Middle Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot - BALB/c Mouse splenocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with BD Horizon RB780 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 557308 and with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465 or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570356/570426; Middle Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot - BALB/c Mouse splenocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with BD Horizon RB780 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Left Plots and stimulated Right Plot Mouse splenic leukocytes. Left and Middle Plots - BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 antibody Cat. No. 557308 and with either BD Horizon RB780 Rat IgG1, kappa Isotype Control Cat. No. 570465 or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570356/570426; Middle Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot - BALB/c Mouse splenocytes were stimulated for 3 days with Concanavalin A ConA . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with BD Horizon RB780 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from DAPI-negative-gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.