BD HorizonTM RB780 Rat Anti-Mouse CD25 (IL-2 Receptor alpha)

Description

Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570767/570845; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570767/570845; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570767/570845; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570767/570845; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570767/570845; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on Unstimulated and Stimulated Mouse splenic leukocytes. Unstimulated Cells Left and Middle Plots : Freshly prepared BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The Unstimulated cells were then stained with APC Rat Anti-Mouse CD4 antibody Cat. No. 553051 and with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control Cat. No. 568699; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody Cat. No. 570767/570845; Middle Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Stimulated Cells Right Plot : Mouse splenic leukocytes were stimulated with Concanavalin A Con A for 3 days. The Stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon RB780 Rat IgG2b, kappa Isotype Control dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD25 IL-2 Receptor alpha antibody solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.