BD HorizonTM RB780 Rat Anti-Mouse CD31

Description

Multicolor flow cytometric analysis of CD31 expression on viable Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD31 antibody Cat. No. 569358/569359; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD31 expression on viable Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD31 antibody Cat. No. 569358/569359; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD31 expression on viable Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD31 antibody Cat. No. 569358/569359; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD31 expression on viable Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD31 antibody Cat. No. 569358/569359; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD31 expression on viable Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD31 antibody Cat. No. 569358/569359; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD31 expression on viable Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553092/561880 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD31 antibody Cat. No. 569358/569359; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD31 or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.