BD HorizonTM RB780 Rat Anti-Mouse CD326

Description

Multiparameter flow cytometric analysis of CD326 expression on BALB/c Mouse thymocytes and splenic T lymphocytes. Left Plot: Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD OptiBuild BV480 Rat Anti-Mouse CD4 Cat. No. 746475 and BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Cat. No. 568736/568737; solid line histogram at 1.0 mug/test. The histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: Mouse splenic leucocytes were preincubated with Mouse BD Fc Block and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Middle Plot or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD326 or Ig Isotype control staining versus CD3e was derived from CD25+ gated events with the light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells did not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD326 expression on BALB/c Mouse thymocytes and splenic T lymphocytes. Left Plot: Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD OptiBuild BV480 Rat Anti-Mouse CD4 Cat. No. 746475 and BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Cat. No. 568736/568737; solid line histogram at 1.0 mug/test. The histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: Mouse splenic leucocytes were preincubated with Mouse BD Fc Block and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Middle Plot or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD326 or Ig Isotype control staining versus CD3e was derived from CD25+ gated events with the light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells did not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD326 expression on BALB/c Mouse thymocytes and splenic T lymphocytes. Left Plot: Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD OptiBuild BV480 Rat Anti-Mouse CD4 Cat. No. 746475 and BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Cat. No. 568736/568737; solid line histogram at 1.0 mug/test. The histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: Mouse splenic leucocytes were preincubated with Mouse BD Fc Block and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Middle Plot or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD326 or Ig Isotype control staining versus CD3e was derived from CD25+ gated events with the light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells did not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD326 expression on BALB/c Mouse thymocytes and splenic T lymphocytes. Left Plot: Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD OptiBuild BV480 Rat Anti-Mouse CD4 Cat. No. 746475 and BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Cat. No. 568736/568737; solid line histogram at 1.0 mug/test. The histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: Mouse splenic leucocytes were preincubated with Mouse BD Fc Block and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Middle Plot or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD326 or Ig Isotype control staining versus CD3e was derived from CD25+ gated events with the light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells did not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD326 expression on BALB/c Mouse thymocytes and splenic T lymphocytes. Left Plot: Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD OptiBuild BV480 Rat Anti-Mouse CD4 Cat. No. 746475 and BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Cat. No. 568736/568737; solid line histogram at 1.0 mug/test. The histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: Mouse splenic leucocytes were preincubated with Mouse BD Fc Block and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Middle Plot or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD326 or Ig Isotype control staining versus CD3e was derived from CD25+ gated events with the light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells did not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD326 expression on BALB/c Mouse thymocytes and splenic T lymphocytes. Left Plot: Mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD OptiBuild BV480 Rat Anti-Mouse CD4 Cat. No. 746475 and BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Cat. No. 568736/568737; solid line histogram at 1.0 mug/test. The histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: Mouse splenic leucocytes were preincubated with Mouse BD Fc Block and stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370 antibodies and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Middle Plot or BD Horizon RB780 Rat Anti-Mouse CD326 antibody Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD326 or Ig Isotype control staining versus CD3e was derived from CD25+ gated events with the light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells did not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.