BD HorizonTM RB780 Rat Anti-Mouse CD86 (B7-2)

Description

Flow cytometric analysis of CD86 B7-2 expression on resting or activated Mouse splenic leucocytes. Freshly isolated Left Plot or 72-hour lipopolysaccharide-stimulated Right Plot BALB/c Mouse splenocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histograms or BD Horizon RB780 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 569219/569220; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative resting Left Plot or activated Right Plot lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD86 B7-2 expression on resting or activated Mouse splenic leucocytes. Freshly isolated Left Plot or 72-hour lipopolysaccharide-stimulated Right Plot BALB/c Mouse splenocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histograms or BD Horizon RB780 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 569219/569220; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative resting Left Plot or activated Right Plot lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD86 B7-2 expression on resting or activated Mouse splenic leucocytes. Freshly isolated Left Plot or 72-hour lipopolysaccharide-stimulated Right Plot BALB/c Mouse splenocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histograms or BD Horizon RB780 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 569219/569220; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative resting Left Plot or activated Right Plot lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD86 B7-2 expression on resting or activated Mouse splenic leucocytes. Freshly isolated Left Plot or 72-hour lipopolysaccharide-stimulated Right Plot BALB/c Mouse splenocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histograms or BD Horizon RB780 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 569219/569220; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative resting Left Plot or activated Right Plot lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD86 B7-2 expression on resting or activated Mouse splenic leucocytes. Freshly isolated Left Plot or 72-hour lipopolysaccharide-stimulated Right Plot BALB/c Mouse splenocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histograms or BD Horizon RB780 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 569219/569220; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative resting Left Plot or activated Right Plot lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD86 B7-2 expression on resting or activated Mouse splenic leucocytes. Freshly isolated Left Plot or 72-hour lipopolysaccharide-stimulated Right Plot BALB/c Mouse splenocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histograms or BD Horizon RB780 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 569219/569220; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative resting Left Plot or activated Right Plot lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.