BD HorizonTM RB780 Rat Anti-Mouse CD8a

Description

Two-color flow cytometric analysis of CD8a expression on Mouse splenocytes. BALB/c mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD8a antibody Cat. No. 568692/568693; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenocytes. BALB/c mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD8a antibody Cat. No. 568692/568693; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenocytes. BALB/c mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD8a antibody Cat. No. 568692/568693; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on Mouse splenocytes. BALB/c mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD8a antibody Cat. No. 568692/568693; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on Mouse splenocytes. BALB/c mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD8a antibody Cat. No. 568692/568693; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenocytes. BALB/c mouse splenic leucocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse CD8a antibody Cat. No. 568692/568693; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.