BD HorizonTM RB780 Rat Anti-Mouse CD8b

Description

Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and BD Horizon RB780 Rat Anti-Mouse CD8b antibody Cat. No. 569478/569479 at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and BD Horizon RB780 Rat Anti-Mouse CD8b antibody Cat. No. 569478/569479 at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric expression of CD8b expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and BD Horizon RB780 Rat Anti-Mouse CD8b antibody Cat. No. 569478/569479 at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD8b versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.