BD HorizonTM RB780 Rat Anti-Mouse Ly-6G

Description

Flow cytometric analysis of Ly-6G expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse Ly-6G antibody Cat. No. 569152/569153; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Ly-6G expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-6G antibody Cat. No. 569152/569153; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-6G expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse Ly-6G antibody Cat. No. 569152/569153; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of Ly-6G expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-6G antibody Cat. No. 569152/569153; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-6G expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; dashed line histogram or BD Horizon RB780 Rat Anti-Mouse Ly-6G antibody Cat. No. 569152/569153; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of Ly-6G or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Ly-6G expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were stained with either BD Horizon RB780 Rat IgG2a, kappa Isotype Control Cat. No. 568698; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-6G antibody Cat. No. 569152/569153; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6G or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.