BD HorizonTM RB780 Rat Anti-Mouse Ly-76 (TER-119)

Description

Two-color flow cytometric analysis of Ly-76 TER-119 expression on Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block antibody Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45 antibody Cat. No. 559864 and with either no antibody Autofluorescence Control; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-76 TER-119 antibody Cat. No. 569365/569366; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-76 TER-119 or Autofluorescence versus CD45 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-76 TER-119 expression on Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block antibody Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45 antibody Cat. No. 559864 and with either no antibody Autofluorescence Control; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-76 TER-119 antibody Cat. No. 569365/569366; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-76 TER-119 or Autofluorescence versus CD45 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-76 TER-119 expression on Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block antibody Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45 antibody Cat. No. 559864 and with either no antibody Autofluorescence Control; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-76 TER-119 antibody Cat. No. 569365/569366; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-76 TER-119 or Autofluorescence versus CD45 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-76 TER-119 expression on Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block antibody Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45 antibody Cat. No. 559864 and with either no antibody Autofluorescence Control; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-76 TER-119 antibody Cat. No. 569365/569366; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-76 TER-119 or Autofluorescence versus CD45 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-76 TER-119 expression on Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block antibody Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45 antibody Cat. No. 559864 and with either no antibody Autofluorescence Control; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-76 TER-119 antibody Cat. No. 569365/569366; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-76 TER-119 or Autofluorescence versus CD45 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-76 TER-119 expression on Mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block antibody Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD45 antibody Cat. No. 559864 and with either no antibody Autofluorescence Control; Left Plot or BD Horizon RB780 Rat Anti-Mouse Ly-76 TER-119 antibody Cat. No. 569365/569366; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-76 TER-119 or Autofluorescence versus CD45 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.