BD HorizonTM RB824 Mouse Anti-Human CD45RO

Description

Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with PE Mouse Anti-Human CD45RA antibody Cat. No. 555489; Bottom Plots and with either BD Horizon RB824 Mouse IgG2a, kappa Isotype Control Cat. No. 572560; Left Plots or BD Horizon RB824 Mouse Anti-Human CD45RO antibody Cat. No. 572554/572555; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and compensated using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing the coexpression of CD45RO or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with PE Mouse Anti-Human CD45RA antibody Cat. No. 555489; Bottom Plots and with either BD Horizon RB824 Mouse IgG2a, kappa Isotype Control Cat. No. 572560; Left Plots or BD Horizon RB824 Mouse Anti-Human CD45RO antibody Cat. No. 572554/572555; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and compensated using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing the coexpression of CD45RO or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with PE Mouse Anti-Human CD45RA antibody Cat. No. 555489; Bottom Plots and with either BD Horizon RB824 Mouse IgG2a, kappa Isotype Control Cat. No. 572560; Left Plots or BD Horizon RB824 Mouse Anti-Human CD45RO antibody Cat. No. 572554/572555; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and compensated using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing the coexpression of CD45RO or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.