BD HorizonTM RB824 Mouse Anti-Human FoxP3

Description

Two-color flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes . Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 563550 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 followed by intracellular staining with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plot or BD Horizon RB824 Mouse Anti-Human FoxP3 antibody Cat. No. 572533/572534; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of FoxP3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes . Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 563550 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 followed by intracellular staining with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plot or BD Horizon RB824 Mouse Anti-Human FoxP3 antibody Cat. No. 572533/572534; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of FoxP3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes . Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 563550 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 followed by intracellular staining with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plot or BD Horizon RB824 Mouse Anti-Human FoxP3 antibody Cat. No. 572533/572534; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of FoxP3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes . Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 563550 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 followed by intracellular staining with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plot or BD Horizon RB824 Mouse Anti-Human FoxP3 antibody Cat. No. 572533/572534; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of FoxP3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes . Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 563550 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 followed by intracellular staining with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plot or BD Horizon RB824 Mouse Anti-Human FoxP3 antibody Cat. No. 572533/572534; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of FoxP3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of FoxP3 expression in resting Human peripheral blood lymphocytes . Human peripheral blood mononuclear cells PBMC were stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 563550 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 followed by intracellular staining with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plot or BD Horizon RB824 Mouse Anti-Human FoxP3 antibody Cat. No. 572533/572534; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of FoxP3 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software.