BD HorizonTM RB824 Mouse Anti-Human KLRG1

Description

Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 569802; Top Plots and with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plots or BD Horizon RB824 Mouse Anti-Human KLRG1 antibody Cat. No. 572550/572551; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 569802; Top Plots and with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plots or BD Horizon RB824 Mouse Anti-Human KLRG1 antibody Cat. No. 572550/572551; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Show More Multiparameter flow cytometric analysis of KLRG1 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 569802; Top Plots and with either BD Horizon RB824 Mouse IgG1, kappa Isotype Control Cat. No. 572537; Left Plots or BD Horizon RB824 Mouse Anti-Human KLRG1 antibody Cat. No. 572550/572551; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Top Plots - The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NCAM-1 CD56 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Bottom Plots - The bivariate pseudocolor density plot showing KLRG1 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software.