BD HorizonTM RB824 Rat Anti-Mouse CD8a

Description

Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB824 Rat IgG2a, kappa Isotype Control Cat. No. 572536; Left Plot or BD Horizon RB824 Rat Anti-Mouse CD8a antibody Cat. No. 572562/572563; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB824 Rat IgG2a, kappa Isotype Control Cat. No. 572536; Left Plot or BD Horizon RB824 Rat Anti-Mouse CD8a antibody Cat. No. 572562/572563; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB824 Rat IgG2a, kappa Isotype Control Cat. No. 572536; Left Plot or BD Horizon RB824 Rat Anti-Mouse CD8a antibody Cat. No. 572562/572563; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB824 Rat IgG2a, kappa Isotype Control Cat. No. 572536; Left Plot or BD Horizon RB824 Rat Anti-Mouse CD8a antibody Cat. No. 572562/572563; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB824 Rat IgG2a, kappa Isotype Control Cat. No. 572536; Left Plot or BD Horizon RB824 Rat Anti-Mouse CD8a antibody Cat. No. 572562/572563; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB824 Rat IgG2a, kappa Isotype Control Cat. No. 572536; Left Plot or BD Horizon RB824 Rat Anti-Mouse CD8a antibody Cat. No. 572562/572563; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.