BD HorizonTM RB824 Rat Anti-Mouse Ly-6C

Description

Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RB824 Rat IgM, kappa Isotype Control Cat. No. 572561; Left Plot or BD Horizon RB824 Rat Anti-Mouse Ly-6C antibody Cat. No. 572557/572558; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RB824 Rat IgM, kappa Isotype Control Cat. No. 572561; Left Plot or BD Horizon RB824 Rat Anti-Mouse Ly-6C antibody Cat. No. 572557/572558; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RB824 Rat IgM, kappa Isotype Control Cat. No. 572561; Left Plot or BD Horizon RB824 Rat Anti-Mouse Ly-6C antibody Cat. No. 572557/572558; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RB824 Rat IgM, kappa Isotype Control Cat. No. 572561; Left Plot or BD Horizon RB824 Rat Anti-Mouse Ly-6C antibody Cat. No. 572557/572558; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RB824 Rat IgM, kappa Isotype Control Cat. No. 572561; Left Plot or BD Horizon RB824 Rat Anti-Mouse Ly-6C antibody Cat. No. 572557/572558; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RB824 Rat IgM, kappa Isotype Control Cat. No. 572561; Left Plot or BD Horizon RB824 Rat Anti-Mouse Ly-6C antibody Cat. No. 572557/572558; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.