BD HorizonTM RR688 Mouse Anti-Human Granzyme B

Description

Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RR688 Mouse IgG1, kappa Isotype Control Cat. No. 572400; Left Plot or BD Horizon RR688 Mouse Anti-Human Granzyme B antibody Cat. No. 572411/572412; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RR688 Mouse IgG1, kappa Isotype Control Cat. No. 572400; Left Plot or BD Horizon RR688 Mouse Anti-Human Granzyme B antibody Cat. No. 572411/572412; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RR688 Mouse IgG1, kappa Isotype Control Cat. No. 572400; Left Plot or BD Horizon RR688 Mouse Anti-Human Granzyme B antibody Cat. No. 572411/572412; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RR688 Mouse IgG1, kappa Isotype Control Cat. No. 572400; Left Plot or BD Horizon RR688 Mouse Anti-Human Granzyme B antibody Cat. No. 572411/572412; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RR688 Mouse IgG1, kappa Isotype Control Cat. No. 572400; Left Plot or BD Horizon RR688 Mouse Anti-Human Granzyme B antibody Cat. No. 572411/572412; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RR688 Mouse IgG1, kappa Isotype Control Cat. No. 572400; Left Plot or BD Horizon RR688 Mouse Anti-Human Granzyme B antibody Cat. No. 572411/572412; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software.