BD HorizonTM RY586 Hamster Anti-Mouse CD3e

Description

Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and BD Horizon BV421 Rat Anti-Mouse CD8a Cat. No. 563898 antibodies and with either BD Horizon RY586 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 568159; Left Plot or BD Horizon RY586 Hamster Anti-Mouse CD3e antibody Cat. No. 568157/568158; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and BD Horizon BV421 Rat Anti-Mouse CD8a Cat. No. 563898 antibodies and with either BD Horizon RY586 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 568159; Left Plot or BD Horizon RY586 Hamster Anti-Mouse CD3e antibody Cat. No. 568157/568158; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and BD Horizon BV421 Rat Anti-Mouse CD8a Cat. No. 563898 antibodies and with either BD Horizon RY586 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 568159; Left Plot or BD Horizon RY586 Hamster Anti-Mouse CD3e antibody Cat. No. 568157/568158; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and BD Horizon BV421 Rat Anti-Mouse CD8a Cat. No. 563898 antibodies and with either BD Horizon RY586 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 568159; Left Plot or BD Horizon RY586 Hamster Anti-Mouse CD3e antibody Cat. No. 568157/568158; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and BD Horizon BV421 Rat Anti-Mouse CD8a Cat. No. 563898 antibodies and with either BD Horizon RY586 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 568159; Left Plot or BD Horizon RY586 Hamster Anti-Mouse CD3e antibody Cat. No. 568157/568158; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and BD Horizon BV421 Rat Anti-Mouse CD8a Cat. No. 563898 antibodies and with either BD Horizon RY586 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 568159; Left Plot or BD Horizon RY586 Hamster Anti-Mouse CD3e antibody Cat. No. 568157/568158; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.