BD HorizonTM RY586 Mouse Anti-Human CD10

Description

Multiparameter flow cytometric analysis of CD10 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD10 antibody Cat. No. 568447/568448; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD10 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD10 expression on human REH cells. Cells from the REH Acute B cell leukemia, ATCC CRL-8283 cell line were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD10 antibody Cat. No. 568447/568448; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD10 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer and FlowJo software Multiparameter flow cytometric analysis of CD10 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD10 antibody Cat. No. 568447/568448; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD10 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD10 expression on human REH cells. Cells from the REH Acute B cell leukemia, ATCC CRL-8283 cell line were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD10 antibody Cat. No. 568447/568448; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD10 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer and FlowJo software Show More Multiparameter flow cytometric analysis of CD10 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD10 antibody Cat. No. 568447/568448; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD10 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD10 expression on human REH cells. Cells from the REH Acute B cell leukemia, ATCC CRL-8283 cell line were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD10 antibody Cat. No. 568447/568448; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD10 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer and FlowJo software