BD HorizonTM RY586 Mouse Anti-Human CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and PHA-stimulated human peripheral blood lymphocytes Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561840/555349/561841 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097 or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on unstimulated and PHA-stimulated human peripheral blood lymphocytes Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561840/555349/561841 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097 or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on unstimulated and PHA-stimulated human peripheral blood lymphocytes Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561840/555349/561841 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097 or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD25 expression on unstimulated and PHA-stimulated human peripheral blood lymphocytes Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561840/555349/561841 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097 or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD25 expression on unstimulated and PHA-stimulated human peripheral blood lymphocytes Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561840/555349/561841 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097 or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on unstimulated and PHA-stimulated human peripheral blood lymphocytes Left and Middle Plots: Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 561840/555349/561841 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097 or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568459/568460; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.