BD HorizonTM RY586 Mouse Anti-Human CD25

Description

Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots- Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescent histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots- Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescent histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots- Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescent histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots- Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescent histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots- Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescent histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots- Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD25 antibody Cat. No. 568882/568883; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescent histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.