BD HorizonTM RY586 Mouse Anti-Human CD45RO

Description

Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RA antibody Cat. No. 550855/561884 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plots or BD Horizon RY586 Mouse Anti-Human CD45RO antibody Cat. No. 568473/568474; Right Plots . Erythrocytes were lysed with BD FACS Lysing Buffer Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Upper Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of intact leucocyte populations. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig isotype control staining versus CD45RA was derived from gated events with the side and forward light-scatter characteristics of intact lymphocytes. Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RA antibody Cat. No. 550855/561884 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plots or BD Horizon RY586 Mouse Anti-Human CD45RO antibody Cat. No. 568473/568474; Right Plots . Erythrocytes were lysed with BD FACS Lysing Buffer Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Upper Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of intact leucocyte populations. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig isotype control staining versus CD45RA was derived from gated events with the side and forward light-scatter characteristics of intact lymphocytes. Show More Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RA antibody Cat. No. 550855/561884 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plots or BD Horizon RY586 Mouse Anti-Human CD45RO antibody Cat. No. 568473/568474; Right Plots . Erythrocytes were lysed with BD FACS Lysing Buffer Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Upper Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of intact leucocyte populations. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig isotype control staining versus CD45RA was derived from gated events with the side and forward light-scatter characteristics of intact lymphocytes.