
569649 · 25 Tests
BDB Bioscience · Cat: 569649
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD63 expression on Human peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin Sigma-Aldrich, Cat. No. T8885 , and then fixed with 2 % formaldehyde. After washing, the fixed platelets were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD63 antibody Cat. No. 569648/569649; solid line histogram . The fluorescence histogram showing CD63 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD63 expression on Human peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin Sigma-Aldrich, Cat. No. T8885 , and then fixed with 2 % formaldehyde. After washing, the fixed platelets were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD63 antibody Cat. No. 569648/569649; solid line histogram . The fluorescence histogram showing CD63 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD63 expression on Human peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin Sigma-Aldrich, Cat. No. T8885 , and then fixed with 2 % formaldehyde. After washing, the fixed platelets were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD63 antibody Cat. No. 569648/569649; solid line histogram . The fluorescence histogram showing CD63 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.