
568496 · 25 Tests
BDB Bioscience · Cat: 568496
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of CD90 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD34 antibody Cat. No. 555824 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD90 antibody Cat. No. 568495/568496; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD90 expression on Human HEL cells. HEL cells Human erythroleukemia cell line; ATCC TIB-180 were stained with BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD90 antibody Cat. No. 568495/568496, solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD90 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD34 antibody Cat. No. 555824 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD90 antibody Cat. No. 568495/568496; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD90 expression on Human HEL cells. HEL cells Human erythroleukemia cell line; ATCC TIB-180 were stained with BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD90 antibody Cat. No. 568495/568496, solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD90 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with APC Mouse Anti-Human CD34 antibody Cat. No. 555824 and with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; Left Plot or BD Horizon RY586 Mouse Anti-Human CD90 antibody Cat. No. 568495/568496; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD90 expression on Human HEL cells. HEL cells Human erythroleukemia cell line; ATCC TIB-180 were stained with BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histogram or BD Horizon RY586 Mouse Anti-Human CD90 antibody Cat. No. 568495/568496, solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.