
571180 · 25 Tests
BDB Bioscience · Cat: 571180
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Description
Two-color flow cytometric analysis of TCR Vbeta11 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human TCR Vbeta11 antibody Cat. No. 571179/571180; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta11 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of TCR Vbeta11 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human TCR Vbeta11 antibody Cat. No. 571179/571180; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta11 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of TCR Vbeta11 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human TCR Vbeta11 antibody Cat. No. 571179/571180; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta11 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of TCR Vbeta11 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human TCR Vbeta11 antibody Cat. No. 571179/571180; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta11 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of TCR Vbeta11 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human TCR Vbeta11 antibody Cat. No. 571179/571180; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta11 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of TCR Vbeta11 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY586 Mouse IgG2a, kappa Isotype Control Cat. No. 568131; Left Plot or BD Horizon RY586 Mouse Anti-Human TCR Vbeta11 antibody Cat. No. 571179/571180; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TCR Vbeta11 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.