BD HorizonTM RY586 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RY586 Mouse IgG1 Isotype Control Cat. No. 568097; Left Plots or BD Horizon RY586 Mouse Anti-Ki-67 antibody Cat. No. 568147/568148; Right Plots and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DAPI staining versus Ki-67 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RY586 Mouse IgG1 Isotype Control Cat. No. 568097; Left Plots or BD Horizon RY586 Mouse Anti-Ki-67 antibody Cat. No. 568147/568148; Right Plots and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DAPI staining versus Ki-67 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RY586 Mouse IgG1 Isotype Control Cat. No. 568097; Left Plots or BD Horizon RY586 Mouse Anti-Ki-67 antibody Cat. No. 568147/568148; Right Plots and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DAPI staining versus Ki-67 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.