BD HorizonTM RY586 Mouse Anti-Mouse H-2Kb/SIINFEKL

Description

Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Panel or pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histograms or BD Horizon RY586 Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 570018/570099; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Panel or pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histograms or BD Horizon RY586 Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 570018/570099; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Panel or pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histograms or BD Horizon RY586 Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 570018/570099; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Panel or pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histograms or BD Horizon RY586 Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 570018/570099; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Panel or pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histograms or BD Horizon RY586 Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 570018/570099; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2Kb/SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Panel or pulsed Right Panel with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either BD Horizon RY586 Mouse IgG1, kappa Isotype Control Cat. No. 568097; dashed line histograms or BD Horizon RY586 Mouse Anti-Mouse H-2Kb/SIINFEKL antibody Cat. No. 570018/570099; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing H-2Kb/SIINFEKL expression or Ig Isotype control expression were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.