BD HorizonTM RY586 Rat Anti-CD11b

Description

Multiparameter flow cytometric analysis of CD11b expression on Mouse bone-marrow cells. Bone-marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD11b antibody Cat. No. 568485/568486; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow cell populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-CD11b antibody Cat. No. 568485/568486; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b expression on Mouse bone-marrow cells. Bone-marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD11b antibody Cat. No. 568485/568486; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow cell populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD11b expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-CD11b antibody Cat. No. 568485/568486; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD11b expression on Mouse bone-marrow cells. Bone-marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD11b antibody Cat. No. 568485/568486; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative bone marrow cell populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-CD11b antibody Cat. No. 568485/568486; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD11b or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.