BD HorizonTM RY586 Rat Anti-Human IL-13

Description

Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 566907 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822, Left Plot or BD Horizon RY586 Rat Anti-Human IL-13 antibody Cat No. 568455/568456, Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a Symphony A5 Cell Analyzer System and FlowJo software. Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 566907 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822, Left Plot or BD Horizon RY586 Rat Anti-Human IL-13 antibody Cat No. 568455/568456, Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a Symphony A5 Cell Analyzer System and FlowJo software. Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 566907 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822, Left Plot or BD Horizon RY586 Rat Anti-Human IL-13 antibody Cat No. 568455/568456, Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a Symphony A5 Cell Analyzer System and FlowJo software. Show More Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 566907 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822, Left Plot or BD Horizon RY586 Rat Anti-Human IL-13 antibody Cat No. 568455/568456, Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a Symphony A5 Cell Analyzer System and FlowJo software. Show More Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 566907 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822, Left Plot or BD Horizon RY586 Rat Anti-Human IL-13 antibody Cat No. 568455/568456, Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a Symphony A5 Cell Analyzer System and FlowJo software. Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 566907 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822, Left Plot or BD Horizon RY586 Rat Anti-Human IL-13 antibody Cat No. 568455/568456, Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a Symphony A5 Cell Analyzer System and FlowJo software.