BD HorizonTM RY586 Rat Anti-Human IL-4

Description

Two-color flow cytometric analysis of IL-4 expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822; Left Plot or BD Horizon RY586 Rat Anti-Human IL-4 antibody Cat. No. 568518/568519; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IL-4 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-4 expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822; Left Plot or BD Horizon RY586 Rat Anti-Human IL-4 antibody Cat. No. 568518/568519; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IL-4 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-4 expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822; Left Plot or BD Horizon RY586 Rat Anti-Human IL-4 antibody Cat. No. 568518/568519; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IL-4 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-4 expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822; Left Plot or BD Horizon RY586 Rat Anti-Human IL-4 antibody Cat. No. 568518/568519; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IL-4 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IL-4 expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822; Left Plot or BD Horizon RY586 Rat Anti-Human IL-4 antibody Cat. No. 568518/568519; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IL-4 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IL-4 expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822; Left Plot or BD Horizon RY586 Rat Anti-Human IL-4 antibody Cat. No. 568518/568519; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IL-4 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.