BD HorizonTM RY586 Rat Anti-Mouse CD326

Description

Two-color flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD326 antibody Cat. No. 568126/568127 at 0.5 mug/test. Left Plot: Thymocytes were further stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies. The fluorescence histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370/566228 antibodies. Two-color flow cytometric contour plots showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e were derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD326 antibody Cat. No. 568126/568127 at 0.5 mug/test. Left Plot: Thymocytes were further stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies. The fluorescence histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370/566228 antibodies. Two-color flow cytometric contour plots showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e were derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD326 antibody Cat. No. 568126/568127 at 0.5 mug/test. Left Plot: Thymocytes were further stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies. The fluorescence histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370/566228 antibodies. Two-color flow cytometric contour plots showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e were derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD326 antibody Cat. No. 568126/568127 at 0.5 mug/test. Left Plot: Thymocytes were further stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies. The fluorescence histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370/566228 antibodies. Two-color flow cytometric contour plots showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e were derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD326 antibody Cat. No. 568126/568127 at 0.5 mug/test. Left Plot: Thymocytes were further stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies. The fluorescence histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370/566228 antibodies. Two-color flow cytometric contour plots showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e were derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD326 antibody Cat. No. 568126/568127 at 0.5 mug/test. Left Plot: Thymocytes were further stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 562891 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies. The fluorescence histogram showing CD326 expression or Ig Isotype control staining was derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 and BD Horizon BV421 Rat Anti-Mouse CD25 Cat. No. 564370/566228 antibodies. Two-color flow cytometric contour plots showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e were derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.