BD HorizonTM RY586 Rat Anti-Mouse CD366 (TIM-3)

Description

Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 570025/570106; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 570025/570106; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 570025/570106; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 570025/570106; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 570025/570106; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD366 TIM-3 expression on viable activated Mouse splenic leukocytes. BALB/c Mouse splenocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057/567114; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 567110/553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD366 TIM-3 antibody Cat. No. 570025/570106; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.