BD HorizonTM RY586 Rat Anti-Mouse CD8a

Description

Two-color flow cytometric analysis of CD8 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142. The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD8a antibody Cat. No. 568163/568164; Right Plot at 0.5 mug/test. The two-color fluorescence contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142. The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD8a antibody Cat. No. 568163/568164; Right Plot at 0.5 mug/test. The two-color fluorescence contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142. The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD8a antibody Cat. No. 568163/568164; Right Plot at 0.5 mug/test. The two-color fluorescence contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142. The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD8a antibody Cat. No. 568163/568164; Right Plot at 0.5 mug/test. The two-color fluorescence contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142. The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD8a antibody Cat. No. 568163/568164; Right Plot at 0.5 mug/test. The two-color fluorescence contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142. The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY586 Rat IgG2a, kappa Isotype Control Cat. No. 568130; Left Plot or BD Horizon RY586 Rat Anti-Mouse CD8a antibody Cat. No. 568163/568164; Right Plot at 0.5 mug/test. The two-color fluorescence contour plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristic of viable splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.