BD HorizonTM RY586 Rat Anti-Mouse I-A/I-E

Description

Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse I-A/I-E antibody Cat. No. 568545/568546 Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse I-A/I-E antibody Cat. No. 568545/568546 Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse I-A/I-E antibody Cat. No. 568545/568546 Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse I-A/I-E antibody Cat. No. 568545/568546 Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse I-A/I-E antibody Cat. No. 568545/568546 Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse I-A/I-E antibody Cat. No. 568545/568546 Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.