BD HorizonTM RY586 Rat Anti-Mouse IL-2

Description

Two color flow cytometric analysis of IL-2 expression in Stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse IL-2 antibody Cat. No. 568543/568544; Right Plot at 0.125 mug/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two color flow cytometric analysis of IL-2 expression in Stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse IL-2 antibody Cat. No. 568543/568544; Right Plot at 0.125 mug/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two color flow cytometric analysis of IL-2 expression in Stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse IL-2 antibody Cat. No. 568543/568544; Right Plot at 0.125 mug/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two color flow cytometric analysis of IL-2 expression in Stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse IL-2 antibody Cat. No. 568543/568544; Right Plot at 0.125 mug/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two color flow cytometric analysis of IL-2 expression in Stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse IL-2 antibody Cat. No. 568543/568544; Right Plot at 0.125 mug/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two color flow cytometric analysis of IL-2 expression in Stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse IL-2 antibody Cat. No. 568543/568544; Right Plot at 0.125 mug/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side-light scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.