BD HorizonTM RY586 Rat Anti-Mouse Zbtb7b

Description

Two-color flow cytometric analysis of Zbtb7b ThPok expression in mouse thymocytes. Mouse thymocytes were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with BD Horizon Rat Anti-Mouse CD4 antibody Cat. No. 562891 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse Zbtb7b ThPok antibody Cat. No. 568128/568129; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Zbtb7b ThPok or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Zbtb7b ThPok expression in mouse thymocytes. Mouse thymocytes were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with BD Horizon Rat Anti-Mouse CD4 antibody Cat. No. 562891 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse Zbtb7b ThPok antibody Cat. No. 568128/568129; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Zbtb7b ThPok or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Zbtb7b ThPok expression in mouse thymocytes. Mouse thymocytes were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with BD Horizon Rat Anti-Mouse CD4 antibody Cat. No. 562891 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse Zbtb7b ThPok antibody Cat. No. 568128/568129; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Zbtb7b ThPok or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Zbtb7b ThPok expression in mouse thymocytes. Mouse thymocytes were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with BD Horizon Rat Anti-Mouse CD4 antibody Cat. No. 562891 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse Zbtb7b ThPok antibody Cat. No. 568128/568129; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Zbtb7b ThPok or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Zbtb7b ThPok expression in mouse thymocytes. Mouse thymocytes were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with BD Horizon Rat Anti-Mouse CD4 antibody Cat. No. 562891 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse Zbtb7b ThPok antibody Cat. No. 568128/568129; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Zbtb7b ThPok or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Zbtb7b ThPok expression in mouse thymocytes. Mouse thymocytes were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with BD Horizon Rat Anti-Mouse CD4 antibody Cat. No. 562891 and with either BD Horizon RY586 Rat IgG2b, kappa Isotype Control Cat. No. 568160; Left Plot or BD Horizon RY586 Rat Anti-Mouse Zbtb7b ThPok antibody Cat. No. 568128/568129; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Zbtb7b ThPok or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.