BD HorizonTM RY586 Rat Anti-TOX

Description

Multiparameter flow cytometric analysis of TOX expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . Thymocytes were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822 or BD Horizon RY586 Rat Anti-TOX antibody Cat. No. 569632/569633 at 0.5 mug/test. Overlapping, colorized TOX expression histograms Right Plot were generated for the corresponding colorized gated thymocyte subsets defined by the correlated expression of CD8a versus CD4 Left Plot as indicated: CD4+CD8a+ Blue line histogram , CD4loCD8alo Green histogram and CD4+CD8alo Purple histogram with Ig Isotype control staining shown for CD4+CD8+ thymocytes Black histogram . Flow cytometry and data analysis were performed on gated events with the side and forward light-scatter characteristics of intact thymocytes using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TOX expression in Human MOLT-4 cells. Cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon RY586 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY586 Rat Anti-TOX antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing TOX expression or Ig Isotype control staining was derived from gated events with the side and forward light-scatter characteristics of intact MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of TOX expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . Thymocytes were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822 or BD Horizon RY586 Rat Anti-TOX antibody Cat. No. 569632/569633 at 0.5 mug/test. Overlapping, colorized TOX expression histograms Right Plot were generated for the corresponding colorized gated thymocyte subsets defined by the correlated expression of CD8a versus CD4 Left Plot as indicated: CD4+CD8a+ Blue line histogram , CD4loCD8alo Green histogram and CD4+CD8alo Purple histogram with Ig Isotype control staining shown for CD4+CD8+ thymocytes Black histogram . Flow cytometry and data analysis were performed on gated events with the side and forward light-scatter characteristics of intact thymocytes using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TOX expression in Human MOLT-4 cells. Cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon RY586 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY586 Rat Anti-TOX antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing TOX expression or Ig Isotype control staining was derived from gated events with the side and forward light-scatter characteristics of intact MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of TOX expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . Thymocytes were then stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies and with either BD Horizon RY586 Rat IgG1, kappa Isotype Control Cat. No. 568822 or BD Horizon RY586 Rat Anti-TOX antibody Cat. No. 569632/569633 at 0.5 mug/test. Overlapping, colorized TOX expression histograms Right Plot were generated for the corresponding colorized gated thymocyte subsets defined by the correlated expression of CD8a versus CD4 Left Plot as indicated: CD4+CD8a+ Blue line histogram , CD4loCD8alo Green histogram and CD4+CD8alo Purple histogram with Ig Isotype control staining shown for CD4+CD8+ thymocytes Black histogram . Flow cytometry and data analysis were performed on gated events with the side and forward light-scatter characteristics of intact thymocytes using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TOX expression in Human MOLT-4 cells. Cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon RY586 Rat IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY586 Rat Anti-TOX antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing TOX expression or Ig Isotype control staining was derived from gated events with the side and forward light-scatter characteristics of intact MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.