BD HorizonTM RY610 Hamster Anti-Mouse KLRG1

Description

Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 562921 and with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251 was used as a representative Hamster IgG Isotype Control; Left Plot or BD Horizon RY610 Hamster Anti-Mouse KLRG1 antibody Cat. No. 571238/571302; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 562921 and with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251 was used as a representative Hamster IgG Isotype Control; Left Plot or BD Horizon RY610 Hamster Anti-Mouse KLRG1 antibody Cat. No. 571238/571302; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 562921 and with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251 was used as a representative Hamster IgG Isotype Control; Left Plot or BD Horizon RY610 Hamster Anti-Mouse KLRG1 antibody Cat. No. 571238/571302; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 562921 and with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251 was used as a representative Hamster IgG Isotype Control; Left Plot or BD Horizon RY610 Hamster Anti-Mouse KLRG1 antibody Cat. No. 571238/571302; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 562921 and with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251 was used as a representative Hamster IgG Isotype Control; Left Plot or BD Horizon RY610 Hamster Anti-Mouse KLRG1 antibody Cat. No. 571238/571302; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 562921 and with either BD Horizon RY610 Hamster IgG1, kappa Isotype Control Cat. No. 571251 was used as a representative Hamster IgG Isotype Control; Left Plot or BD Horizon RY610 Hamster Anti-Mouse KLRG1 antibody Cat. No. 571238/571302; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.