BD HorizonTM RY610 Mouse Anti-Human Fetal Hemoglobin

Description

Flow cytometric analysis of Fetal Hemoglobin expression in fetal cord blood erythrocytes. Fetal cord blood erythrocytes were fixed 10 min; room temperature with 0.05 % cold glutaraldehyde and then permeabilized with 0.1 % Triton X-100 10 min, RT . The cells were then stained with either BD Horizon RY610 Mouse IgG1, kappa Isotype Control Cat. No. 571151; dashed line histogram or BD Horizon RY610 Mouse Anti-Human Fetal Hemoglobin antibody Cat. No. 571208/571212; solid line histogram . The fluorescence histogram showing Fetal Hemoglobin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact erythrocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Fetal Hemoglobin expression in fetal cord blood erythrocytes. Fetal cord blood erythrocytes were fixed 10 min; room temperature with 0.05 % cold glutaraldehyde and then permeabilized with 0.1 % Triton X-100 10 min, RT . The cells were then stained with either BD Horizon RY610 Mouse IgG1, kappa Isotype Control Cat. No. 571151; dashed line histogram or BD Horizon RY610 Mouse Anti-Human Fetal Hemoglobin antibody Cat. No. 571208/571212; solid line histogram . The fluorescence histogram showing Fetal Hemoglobin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact erythrocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of Fetal Hemoglobin expression in fetal cord blood erythrocytes. Fetal cord blood erythrocytes were fixed 10 min; room temperature with 0.05 % cold glutaraldehyde and then permeabilized with 0.1 % Triton X-100 10 min, RT . The cells were then stained with either BD Horizon RY610 Mouse IgG1, kappa Isotype Control Cat. No. 571151; dashed line histogram or BD Horizon RY610 Mouse Anti-Human Fetal Hemoglobin antibody Cat. No. 571208/571212; solid line histogram . The fluorescence histogram showing Fetal Hemoglobin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact erythrocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.