BD HorizonTM RY610 Rat Anti-Human IL-13

Description

Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Mouse Anti-Human CD4 antibody Cat. No. 566911 and with either BD Horizon RY610 Rat IgG1, kappa Isotype Control Cat. No. 571676; Left Plot or BD Horizon RY610 Rat Anti-Human IL-13 antibody Cat. No. 571260/571320; Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5 Cell Analyzer System and FlowJo Software. Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Mouse Anti-Human CD4 antibody Cat. No. 566911 and with either BD Horizon RY610 Rat IgG1, kappa Isotype Control Cat. No. 571676; Left Plot or BD Horizon RY610 Rat Anti-Human IL-13 antibody Cat. No. 571260/571320; Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5 Cell Analyzer System and FlowJo Software. Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Mouse Anti-Human CD4 antibody Cat. No. 566911 and with either BD Horizon RY610 Rat IgG1, kappa Isotype Control Cat. No. 571676; Left Plot or BD Horizon RY610 Rat Anti-Human IL-13 antibody Cat. No. 571260/571320; Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5 Cell Analyzer System and FlowJo Software. Show More Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Mouse Anti-Human CD4 antibody Cat. No. 566911 and with either BD Horizon RY610 Rat IgG1, kappa Isotype Control Cat. No. 571676; Left Plot or BD Horizon RY610 Rat Anti-Human IL-13 antibody Cat. No. 571260/571320; Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5 Cell Analyzer System and FlowJo Software. Show More Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Mouse Anti-Human CD4 antibody Cat. No. 566911 and with either BD Horizon RY610 Rat IgG1, kappa Isotype Control Cat. No. 571676; Left Plot or BD Horizon RY610 Rat Anti-Human IL-13 antibody Cat. No. 571260/571320; Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5 Cell Analyzer System and FlowJo Software. Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma, Cat. No. P-8139; 50 ng/ml and Ionomycin Sigma, Cat. No. I-0634; 1 mug/ml in the presence of GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Mouse Anti-Human CD4 antibody Cat. No. 566911 and with either BD Horizon RY610 Rat IgG1, kappa Isotype Control Cat. No. 571676; Left Plot or BD Horizon RY610 Rat Anti-Human IL-13 antibody Cat. No. 571260/571320; Right Plot using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5 Cell Analyzer System and FlowJo Software.