BD HorizonTM RY610 Rat Anti-Mouse CD279 (PD-1)

Description

Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenocytes were then stained with either BD Horizon RY610 Rat IgG2a, kappa Isotype Control Cat. No. 571152; dashed line histograms or BD Horizon RY610 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 571237/571301; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenocytes were then stained with either BD Horizon RY610 Rat IgG2a, kappa Isotype Control Cat. No. 571152; dashed line histograms or BD Horizon RY610 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 571237/571301; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenocytes were then stained with either BD Horizon RY610 Rat IgG2a, kappa Isotype Control Cat. No. 571152; dashed line histograms or BD Horizon RY610 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 571237/571301; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenocytes were then stained with either BD Horizon RY610 Rat IgG2a, kappa Isotype Control Cat. No. 571152; dashed line histograms or BD Horizon RY610 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 571237/571301; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenocytes were then stained with either BD Horizon RY610 Rat IgG2a, kappa Isotype Control Cat. No. 571152; dashed line histograms or BD Horizon RY610 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 571237/571301; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The splenocytes were then stained with either BD Horizon RY610 Rat IgG2a, kappa Isotype Control Cat. No. 571152; dashed line histograms or BD Horizon RY610 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 571237/571301; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.